Životopis/Biography Ivo Kalajzic

Official Contact Inforamtion Institution: University of Connecticut Health Center Department Name: Department of Genetics and Developmental Biology Position : Instructor in Basic Research Street And Number : 263 Farmington Avenue ,  Farmington, CT. MC3301 ,  United States Official Phone ,  Official Fax : (860) 679 2461 ,  (860) 679 8345 E-mail ikalaj@neuron.uchc.edu Web   clinical chemistry and laboratory medicine (fundamental medical sciences.Biomedicine and Public Health) Education 1996 M.D. Zagreb University School of Medicine ,  Zagreb ,  Hrvatska (Croatia) Employment Positions 2002 Instructor Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT ,  Farmington ,  United States 2000 - 2002 Postdoctoral fellow Department of Genetics and Developmental Biology University of Connecticut Health Center, Farmington, CT. ,  Farmington ,  United States 1997 - 2000 Postdoctoral fellow Department of Pediatrics University of Connecticut Health Center, Farmington, CT. ,  Farmington ,  United States 1996 - 1997 Intern University Hospital Split, ,  Split ,  Hrvatska (Croatia) Academic Positions Teaching Guest teacher Congres 2003 The Osteoblast Lineage in The OIM Model. Conference: New Research Strategies in Osteogenesis Imperfecta ,  Hospital for Special Surgery. New York ,  ,  New York ,  United States 2002 Use of Col1a1 Transgenic To Identify Populations of Cells Within the Ostaoblast Lineage ,  Dept. of Orthopedics, Creighton University, Omaha. ,  ,  Omaha ,  2000 Dept. of Research, Saint Francis Hospital, Hartford, CT ,  Use of Promoter-Transgenes Mice To Identify Stages Within the Ostaoblast Lineage. ,  ,  Hartford ,  United States 1999 7th International Conference on osteogenesis imperfecta, Montreal, ,  Osteoblastic activity in the OIM mouse ,  ,  Montreal, ,  Canada Projects 2004 - 2005 PI: Kalajzic, I. Title: “Calcifying Cells in the Atherosclerostic Lessions; Ontogeny and Mechanism” ,  Patterson Trust Foundation ,  ,  2002 - 2005 Co-PI:Kalajzic, I Title: “The osteoprogenitor lineage”, Project 1: Analyzing lineage in vitro. ,  NIH-NIDDK U01 Progenitor Cell Genome Anatomy Project ,  ,  2002 - 2004 Current grant support: PI: Kalajzic, I. Title: “Performance of osteoblasts lineage in the OIM mice”. ,  Children brittle bone foundation ,  ,  1998 - 2000 Completed grant support: PI: Kalajzic, I. Title: “Retroviral Targeting of the Osteoprogenitor Cells” ,  Osteogenesis Imperfecta Foundation ,  ,  Publications Articles Ivo   Kalajzic Yang, W. ; Lu, Y. ; Harris, M.A. ; Gluhak-Heinrich, J. ; Bonewald, L.F. : Feng, J.Q. ; Rowe, D.W. ; Harris, S.E. Identification of An Osteocyte-Specific, Mechanically Regulated Region of the Dentin Matrix Protein 1 promoter ,  ASBMR, ABSTRACT ,  2004 Ivo   Kalajzic Suriyapperuma, S.P., Glynn, J., Boucher, N., Kaur, A., Krueger, W. and Rowe, D.W. Transcriptional Profiling of Murine Marrow Stromal Fibroblast Cultures Treated with Noggin ,  ASBMR, ABSTRACT ,  2004 Ivo   Kalajzic Kronenberg, M.S. ; Jiang, X. ; Li, H. ; Pan, L.C. ; Rowe, D.W. Adipocyte Directed Expression of GFP in Transgenic Mice ,  ASBMR, ABSTRACT ,  2004 Ivo   Kalajzic Stahl, A. ; Yang, W. ; Gupta, R. ; Garg, S. ; Achenie, L. ; Feyen, J. ; Rowe, D.W. Microarray Validation of Osteoblast Lineage Differentiation by GFP Marker Genes ,  ASBMR, ABSTRACT. ,  2004 Ivo   Kalajzic Braut, A. ; Guo D. ; Xi, J. ; Kronenberg, M.S. ; Mina, M. ; Harris MA : Harris, S.E. ; Rowe, D.W. Dentin Matrix Protein 1 Expression During Osteoblastic Differentiation: generation of an osteocyte GFP-transgene ,  Bone. ,  2004 ,  35 74-82 Ivo   Kalajzic Bilic, I. ; Kronenberg, M.S. ; Jiang, X. ; Rowe, D.W. Availability of Promoter-GFP Transgenic Mice for Identifying Subpopulations of Cells within the Osteoprogenitor Lineage ,  JBMR, Suppl 1. ,  2003 ,  18 S337 Ivo   Kalajzic Jiang, X. ; Mack, K. ; Delaney, J. ; D. Rowe, D.W. Osteoblast Lineage Differentiation Potential in the OIM Mice ,  JBMR, Suppl 1. ,  2003 ,  18 S229 Ivo   Kalajzic Stahl, A. ; Yang, W. ; Wu, S. ; Johnson, S. ; Feyen, J. ; Rowe, D.W. Microarray Analysis of Subpopulations of Cells Within Differentiating Murine Osteoblast Cultures ,  JBMR, Suppl 1. ,  2003 ,  18 S139 Ivo   Kalajzic Stahl, A. ; Yang, W. ; Wu, S. ; Johnson, S. ; Feyen, J. ; Rowe, D.W. Microarray Analysis of Subpopulations of Cells Within Differentiating Murine Osteoblast Cultures ,  JBMR, Suppl 1. ,  2003 ,  18 S139 Ivo   Kalajzic Katavic, V. ; Grcevic, D. ; Lukic, I.K. ; Vucenik, V. ; Kovacic, N. ; Marusic, A. Non-functional Fas ligand increases the formation of cartilage early in the endochondral bone induction by rhBMP-2. ,  Life Sciences. ,  2003 ,  74 13-28 Ivo   Kalajzic Delany, A.M. ; Bradshaw, A.D. ; Sage, E.H. ; Canalis, E. Osteonectin-null mutation compromises osteoblasts formation, maturation and survival ,  Endocrinology ,  144 ,  2003 ,  (6) 2588-2596 Ivo   Kalajzic Rajan, T.V. ; Paciorkowski, N. ; Kalajzic, I. ; McGuiness, I. Ascorbic acid is a requirement for the morphogenesis of the human filarial parasite Brugia malayi ,  J of Parasitology ,  89 ,  2003 ,  (4) 868-870 Ivo   Kalajzic Kalajzic Z. ; Hurley, M.M. ; Lichtler A.C. ; Rowe D.W. Stage specific inhibition of osteoblast lineage differentiation by FGF2 and noggin ,  J of Cell Biochem. ,  88 ,  2003 ,  (6) 1168-1176 Ivo   Kalajzic Chung, U. ; Stover, M. ; Rowe, D. W. ; Lichtler. A.C. Use of a Col1a1-GFP Retroviral Marker System to Monitor In Vitro Differentiation of ES Cells into Osteoblastic Cells ,  JBMR, Suppl 1. ,  2002 ,  17 S168 Ivo   Kalajzic Erceg, I. ; Marijanovic, I. ; Kronenberg, M.S. ; Tadic, T. ; Lichtler A. C. Dlx5 Stimulates Osteoblast Differentiation in the Presence of Noggin, an Inhibitor of BMP Signaling ,  JBMR, Suppl 1 ,  2002 ,  17 S302 Ivo   Kalajzic Braut, A. ; Stover, M. ; Narayanan, A. ; George, A. ; Mina M. ; Rowe. D.W. Analysis of DMP1 Expression During Osteoblast Lineage Differentiation ,  JBMR, Suppl 1. ,  2002 ,  17 S247 Ivo   Kalajzic Kalajzic, Z. ; Liu, P. ; Du, Z. ; Braut, A. ; Mina, M. ; Canalis, E. ; Rowe, D.W. Directing the expression of a GFP transgene in differentiated osteoblasts: Comparison between collagen and osteocalcin promoters ,  Bone ,  31 ,  2002 6 654-660. Ivo   Kalajzic Terzic, J. , Rumboldt, Z. ; Mack, K. ; Naprta, A. ; Ledgard, F. ; Gronowicz, G. ; Clark, S.H. ; Rowe, D.W. Osteoblastic Response to the Defective Matrix in the Osteogenesis Imperfecta Murine (OIM) Mouse ,  Endocrinology ,  143 (5) 1594-1601 ,  2002 ,  (5) 1594-1601 Ivo   Kalajzic Braut, A. ; Kalajzic, Z. ; Rowe, D.W. ; Kollar, E.J. ; Mina M. Col1a1-GFP transgene expression in developing incisors ,  Connect Tissue Res. ,  43 ,  2002 ,  (2-3) 216-9 Ivo   Kalajzic Kalajzic, Z. ; Kaliterna, M. ; Gronowicz, G. ; Clark, S.H. ; Lichtler, A.C. ; Rowe, D.W. Use of Col1a1GFP transgenes to identify subpopulations of cells at different stages of the osteoblast lineage ,  J Bone Miner Res. ,  2002 ,  17 15-25 Ivo   Kalajzic Kalajzic, Z. ; Liu, P. ; Du, Z. ; Braut, A. ; Canalis, E. ; Derynck, M. ; Mina M. ; Rowe D.W. Analysis of an osteocalcin GFP reporter transgene in transgenic mice ,  JBMR, Suppl 1, ,  2001 ,  16 S368 Ivo   Kalajzic Braut, A. ; Kalajzic, Z. ; Kollar, E.J. ; Rowe, D.W. ; Mina, M. Expression of the Col1a1 Promoter/Green fluorescent protein transgenes during odontoblast differentiation ,  JBMR, Suppl 1 ,  2001 ,  16 S442 Ivo   Kalajzic Lichtler, A.C., Rowe, D.W. Isolation and Characterization of Subpopulations of Cells within the Osteoblast Lineage ,  JBMR, Suppl 1 ,  2001 ,  16 S367 Ivo   Kalajzic Primorac D. ; Rowe D.W. ; Mottes M. ; Barisic I. ; Anticevic D. ; Mirandola S. ; Gomez LIra M. ; Kusec V. ; Glorieux FH. Osteogenesis Imperfecta at the beginning of bone and joint decade ,  Croat. Med. J. ,  42 ,  2001 ,  (4) 392-414 Ivo   Kalajzic Visnjic, D. ; Gronowicz, G., Clark, S.H. ; Lichtler, A.C. ; Rowe, D.W. Col2.3∆TK transgenic mice: a model for conditional ablation of the osteoblast lineage. ,  J Bone Miner Res. ,  2001 ,  16 2222-2232. Ivo   Kalajzic Liu, P ; Stover ML. ; Rowe D.W ; Lichtler A.C. Human bone marrow stromal cells are efficiently transduced by VSV pseudotyped retrovectors without affecting subsequent osteoblastic differentiation ,  Bone ,  29 ,  2001 4 331-335 Ivo   Kalajzic Dacic S. ; Visnjic D. ; Lichtler A.C. ; Rowe D.W. COl1A1 driven transgenic markers of osteoblast lineage progression ,  J Bone Miner Res. ,  2001 ,  16 1228-1236 Ivo   Kalajzic Stover ML. ; Liu P. ; Rowe D.W. ; Lichtler A.C. Use of VSV-G pseudotyped retroviral vectors to target murine osteoprogenitor cells ,  Virology ,  2001 ,  284 37-45 Ivo   Kalajzic Stover ML. ; Wang CH.L. ; McKinstry M. ; Gronowicz G. ; Clark S.H.; Rowe D.W. ; Lichtler A.C. Bone directed expression of Col1a1 promoter driven self-inactivating retroviral vector in bone marrow cells and in transgenic mice ,  Molecular Therapy ,  2001 3 543-550 Ivo   Kalajzic Kalajzic Z. ; Hurley M.M. ; Rowe D.W. Stage specific inhibition of osteoprogenitor lineage progression by FGF2 and noggin ,  JBMR, Suppl 1 ,  2000 ,  15 S216 Ivo   Kalajzic Delany A. ; Canalis E. Aberrant in vitro differentiation of marrow stromal cells from osteonectin-null mice correlates with osteopenic phenotype in vivo ,  JBMR, Suppl 1 ,  2000 ,  15 S344 Ivo   Kalajzic Woitge, H.W. ; Harrison J.R. ; Ivkosic A. ; Clark S.H. ; Krozowski Z. ; Kream B.E. Loss of glucocorticoid function in osteoblast by targeted overexpression of 11ß-hydroxysteroid dehydrogenase type II ,  JBMR, Suppl 1 ,  2000 ,  15 S178 Ivo   Kalajzic Kalajzic Z. ; Visnjic D. ; Gronowicz G ; Clark S.H. ; Rowe D.W. A model for observing osteoblast lineage progression in vivo ,  JBMR, Suppl 1. ,  2000 ,  15 S196 Ivo   Kalajzic Marusic A. ; Grcevic D. ; Katavic V. ; Kovacic N. ; Lukic I.K. ; Lorenzo J.A. Role of B-lymphocytes in new bone formation ,  Laboratory Investigation ,  2000 ,  80 1761-1774 Ivo   Kalajzic Niu Q.T. ; Visnjic D. ; Kalajzic Z. ; Clark S.H. ; Yeh J. ; Rowe D.W. Responsiveness of type I collagen CAT transgene in mice subjected to isometric mechanical loading of bone ,  JBMR, Suppl 1, ,  1999 ,  14 S165 Ivo   Kalajzic Kaliterna M. ; Mallico E. ; Nahounou M. ; Kalajzic Z. ; Clark S.H. ; Gronowicz G. ; Lichtler A.C. ; Rowe D.W. Use of Col1a1GFP transgenes to identify osteoblastic lineage ,  JBMR, Suppl 1 ,  1999 ,  14 S165 Ivo   Kalajzic Liu P. ; Stover M.L. ; Rowe D.W. ; Lichtler A.C. Human bone marrow stromal cell are targeted VSV pseudotyped retrovectors ,  Bone ,  1998 ,  23 S616 Ivo   Kalajzic Visnjic D. ; Naprta A. ; Gronowicz G. ; Clark S.H. ; Rowe D.W. Characterization of the Col2.3∆TK transgenic mouse ,  Bone ,  1998 ,  23 S181 Ivo   Kalajzic Terzic J. ; Mack K. ; Visnjic D. ; Naprta A. , Gronowicz G. ; Clark S.H. ; Rowe D.W. Osteoblastic activity in the OIM mouse. ,  Bone ,  1998 ,  23 S461 Ivo   Kalajzic Visnjic D., Liu P., Stover M.L., Hurley M.M., Rowe D.W., Lichtler A.C. Use of VSV pseudotyped retrovectors to target murine osteoprogenitor cell. ,  Bone 23:S532 1998 ,  1998 ,  23 S532 Ivo   Kalajzic Miric D. ; Rumboldt Z. ; Gancevic I. ; Eterovic D. ; Bozic I. ;Vukovic I., Dynamics of platelet aggregation in acute myocardial infarction ,  Lij. Vijesnik ,  1996 ,  118 198-201 Serials Ivo   Kalajzic The Osteoblast Lineage in The OIM Model ,  Conference: New Research Strategies in Osteogenesis Imperfecta ,  Hospital for Special Surgery ,  New York 2003 Ivo   Kalajzic Rowe D.W. What have we learned from the murine models of OI? Frontiers in molecular and cellular medicine ,  ,  Dubrovnik, Croatia 2001 Ivo   Kalajzic Woitge, H.W. ; Kronenberg, M.S. ; Clark, S.H. ; Gronowicz, G. ; Lichtler, A. C. ; Kream, B.E. Development and characterization of Col1a1-Cre transgenic mice for osteoblast directed genomic recombination ,  IBMS-ECT ,  2001 Ivo   Kalajzic Woitge, H.W. ; Clark, S.H. ; Gronowicz, G. ; Krozowski, Z. ; Harrison, J.R. ; Kream B.E. Inactivation of glucocorticoids in osteoblasts by targeted overexpression of 11ß-xydroxysteroid dehydrogenase type 2 in transgenic mice ,  IBMS-ECT ,  2001 Ivo   Kalajzic Rowe, D.W. ; Kalajzic, Z. ; Liu, P. ; Lichtler, A.C. A strategy of somatic gene therapy for ostegenesis imperfecta, (OI) ,  11th International Congress of Endocrinology, Abstract 9 ,  ,  Sidney 2000 Ivo   Kalajzic Woitge HW ; Kronenberg MS ; Kalajzic I ; Clark S ; Lichtler A ; Kream BE. Development of transgenic mouse lines expressing Col1a1-Cre transgenes as a model for bone-directed gene disruption ,  Z Rheumatol (Suppl.), O63 ,  ,  (Osteologie 2000 in Würzburg) 2000 Ivo   Kalajzic Kalajzic Z. , Kaliterna M. ; Rowe D.W. Use of Col1a1GFP transgenes to identify subpopulation of cells within the osteoblast lineage ,  7th International Conference on osteogenesis imperfecta ,  ,  Montreal, Canada 1999 Ivo   Kalajzic Rowe D.W. ; Lichtler A.C. ; Visnjic D. ; Kalajzic Z. ; Liu P. ; Stover ML. Steps in developing a strategy of somatic gene therapy for murine OI ,  7th International Conference on osteogenesis Imperfecta ,  ,  Montreal, Canada 1999 Ivo   Kalajzic Terzic J. ; Mack K. ; Visnjic D. ; Naprta A. ; Gronowicz G. ; Clark S.H. ; Rowe D.W. Osteoblastic activity in the OIM mouse ,  7th International Conference on osteogenesis Imperfecta ,  ,  Montreal, Canada 1999 Ivo   Kalajzic Visnjic D. ; Liu P. ; Stover M.L. ; Rowe D.W. ; Lichtler A.C. Use of VSV- G pseudotyped retrovectors to target osteoprogenitor cell ,  East Coast Connective Tissue Society Meeting ,  ,  Washington DC 1999 Ivo   Kalajzic Terzic J. ; Mack K. ; Visnjic D. ; Naprta A. ; Gronowicz G. ; Clark S.H. ; Rowe D.W. Osteoblastic activity in the OIM mouse ,  East Coast Connective Tissue Society Meeting ,  ,  Washington DC 1999 Ivo   Kalajzic Niu Q.T. ; Visnjic D. ; Clark S.H. ; Yeh J. ; Rowe D.W. Transgenic markers of lineage progression in mechanically loaded bones ,  East Coast Connective Tissue Society Meeting ,  ,  Washington DC 1999 Ivo   Kalajzic Liu P. ; Stover M.L. ; Rowe D.W. ; Lichtler A.C. Human bone marrow stromal cells targeted by VSV pseudotyped retrovectors maintain their osteoblastic phenotype ,  East Coast Connective Tissue Society Meeting ,  ,  Washington DC 1999 Ivo   Kalajzic Visnjic D. ; Naprta A. ; Gronowicz G. ; Clark S.H. ; Rowe D.W. Conditional ablation of osteoblast lineage cells in Col2.3∆TK trangenic mice ,  East Coast Connective Tissue Society Meeting ,  ,  Washington DC, 1999 Ivo   Kalajzic Terzic J. ; Mack K. ; Visnjic D. ; Naprta A. ; Gronowicz G. ; Clark S.H. ; Yeh J. ; Rowe D.W. Transgenic markers of lineage progression in mechanically loaded bones ,  Proceedings. First Biennial Space Biomedical Investigators Workshop ,  ,  League City, Texas 1999 ,  214 Ivo   Kalajzic Tadic T. ; Kaliterna M. ; Kronenberg M.S. ; Lichtler A.C. Use of replication competent avian retroviral vectors to express proteins in mouse osteoblastic cells ,  East Coast Connective Tissue Society Meeting ,  ,  Philadelphia 1998 Ivo   Kalajzic Liu P. ; Stover M.L. ; Visnjic D. ; Rowe D.W. ; Lichtler A.C. Use of VSV pseudotyped retrovectors to target marrow stromal cells ,  East Coast Connective Tissue Society Meeting ,  ,  Philadelphia 1998 Ivo   Kalajzic Braut, A. ; Kalajzic Z. ; Rowe, D.W. ; Kollar E.J. ; M. Mina Transgenic Mice Expressing the Col1a1-tagged Green Fluorescent Protein in Odontoblasts ,  TMD (June 16-21, 2001) ,  2001 ,  LYON, FRANCE 0 Awards 1999 Outstanding presentation award, East Coast Connective Tissue Society Meeting, Washington DC, ,  Washington ,  United States 2000 Michael Geisman Fellowship Award (Osteogenesis Imperfecta Foundation) ,  ,  2001 ASBMR “John Haddad Young Investigator Award”, ,  2002 Children Brittle Bone Fellowship Award ,  AMAC ---- Memberships All :: :: - :: :: member ,  American Society for Bone and Mineral Research, ,  ,  Pool   YES Cooperation   Znanstvena suradnja;Suradnja sa gospodarstvom;   Expertise Basic Research in Bone Biology Mechanisms and treatments approaches for osteoporosis Osteogenesis Imperfecta Vascular calcification in atherosclerosis My research is focused on addressing the questions related to osteoblast lineage differentiation in the states of pathological disorders of bone (osteoporosis and osteogenesis imperfecta), and in the field of ectopic calcification (vascular calcification). I am currently analyzing the performance of osteoblast lineage in the murine model of osteogenesis imperfecta using the promoter-transgene (green fluorescent protein-GFP) approach to address the questions of differentiation of osteoblast lineage, Recently, I have begun to develop the GFP-lineage based concept to identify the origins of a cell that gives rise to the cells with osteoblastic phenotype (calcifying vascular cells) during the process of vascular calcifications, an as yet undefined population of cells which reside in the arterial vessel wall have the ability to differentiate into cells with the characteristics proper to a mature and functional osteoblast.